PARP1 Antibody

• Background:

  This gene encodes a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

• NCBI Gene ID:

  142

• Swiss Prot:

  P09874

• Accession Number:

  P09874

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-618 amino acids from the Central region of human PARP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Obesity

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  113 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PARP1

• NCBI Official Name:

  Poly [ADP-ribose] polymerase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Majewski, P.M., et al. J. Biol. Chem. 285 (45) :34828-34838 (2010)

• Background Reference 02:

  Kim, M., et al. Cancer Sci. 101 (11) :2436-2442 (2010)

• Background Reference 03:

  Dong, Y., et al. Cancer Res. 70 (20) :8088-8096 (2010)

• Background Reference 04:

  Krishnakumar, R., et al. Mol. Cell 39 (5) :736-749 (2010)

• Other Product Names:

  Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD (+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL

• Tested Applications:

  WB

• Protein ID:

  130781

• Physical Properties:

  Liquid