PARP1 Antibody

• Background:

  PARP1 is a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

• NCBI Gene ID:

  142

• Swiss Prot:

  P09874

• Accession Number:

  P09874

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PARP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-214 amino acids from the N-terminal region of human PARP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Obesity

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  113 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  PARP1

• NCBI Official Name:

  Poly [ADP-ribose] polymerase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Tao, Z., Biochemistry 47 (21), 5804-5813 (2008)

• Background Reference 02:

  Gao, R., Mol. Cancer Ther. (5), 1246-1250 (2008)

• Background Reference 03:

  Schreiber, V., EMBO J. 11 (9), 3263-3269 (1992)

• Other Product Names:

  Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD (+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1, PARP1, ADPRT, PPOL

• Tested Applications:

  WB, IF, Flow

• Protein ID:

  130781

• Physical Properties:

  Liquid