PARP1 Antibody

• Background:

  PARP1 encodes a chromatin-associated enzyme, poly (ADP-ribosyl) transferase, which modifies various nuclear proteins by poly (ADP-ribosyl) ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. PARP1 can be cleaved resulting in fragements of 29 and 85 kDa.

• NCBI Gene ID:

  57097

• Swiss Prot:

  P09874

• Accession Number:

  NP_001609

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PARP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Apoptosis

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PARP1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  113 kDa

• Applications Notes:

  PARP1 antibody can be used for detection of PARP1 by ELISA at 1:62500. PARP1 antibody can be used for detection of PARP1 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PARP11

• NCBI Official Name:

  Poly (ADP-ribose) polymerase family, member 11

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  P, et al., (2004) J. Clin. Invest. 114 (8), 1072-1081.

• Other Product Names:

  PARP1, ARTD11, MIB006, C12orf6

• Tested Applications:

  ELISA, WB

• Protein ID:

  156523968

• Physical Properties:

  Liquid