ACO1 Antibody

• Background:

  Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.

• NCBI Gene ID:

  48

• Swiss Prot:

  P21399

• Accession Number:

  P21399

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  This ACO1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 354-388 amino acids from the Central region of human ACO1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Chicken

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  98 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:2000

• Symbol:

  ACO1

• NCBI Official Name:

  Cytoplasmic aconitate hydratase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hirling H., et al.Nucleic Acids Res. 20:33-39 (1992) .

• Background Reference 02:

  Humphray S.J., et al.Nature 429:369-374 (2004) .

• Background Reference 03:

  Mural R.J., et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

• Background Reference 04:

  Rouault T.A., et al.Proc. Natl. Acad. Sci. U.S.A. 87:7958-7962 (1990) .

• Other Product Names:

  Cytoplasmic aconitate hydratase, Aconitase, 4.2.1.3, Citrate hydro-lyase, Ferritin repressor protein, Iron regulatory protein 1, IRP1, Iron-responsive element-binding protein 1, IRE-BP 1, ACO1, IREB1

• Tested Applications:

  WB

• Protein ID:

  3123225

• Physical Properties:

  Liquid