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HIST2H3A Antibody

CAT: 0223-60-649Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-60-649Size:400 µL
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Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq].
NCBI Gene ID
126961333932653000
Swiss Prot
Q71DI3
Accession Number
Q71DI3
Host
Rabbit
Reactivity
Human
Immunogen
This HIST2H3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-136 amino acids from the C-terminal region of human HIST2H3A.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat, Chicken, Zebrafish, Xenopus, C. Elegans, Drosophila, Pig, Rabbit
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
15 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
HIST2H3A
NCBI Official Name
Histone H3.2
NCBI Organism
Homo sapiens
Background Reference 01
Neumann, H., et al. Mol. Cell 36 (1) :153-163 (2009)
Background Reference 02
Hurd, P.J., et al. J. Biol. Chem. 284 (24) :16575-16583 (2009)
Background Reference 03
Yuan, J., et al. Cell Cycle 8 (11) :1747-1753 (2009)
Background Reference 04
Chang, Q., et al. J. Hepatol. 50 (2) :323-333 (2009)
Other Product Names
Histone H32, Histone H3/m, Histone H3/o, HIST2H3A
Tested Applications
WB
Protein ID
74758899
Physical Properties
Liquid

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