HIST3H3 Antibody

• Background:

  Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq].

• NCBI Gene ID:

  8290

• Swiss Prot:

  Q16695

• Accession Number:

  Q16695

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HIST3H3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the Central region of human HIST3H3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  16 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  HIST3H3

• NCBI Official Name:

  Histone H3.1t

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Tachiwana, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (23) :10454-10459 (2010)

• Background Reference 02:

  Nair, S.S., et al. EMBO Rep. 11 (6) :438-444 (2010)

• Background Reference 03:

  Rampakakis, E., et al. J. Cell. Biochem. 108 (2) :400-407 (2009)

• Background Reference 04:

  Mochizuki, K., et al. Biochem. Biophys. Res. Commun. 371 (2) :324-327 (2008)

• Other Product Names:

  Histone H31t, H3/t, H3t, H3/g, HIST3H3, H3FT

• Tested Applications:

  WB, Flow

• Protein ID:

  18202512

• Physical Properties:

  Liquid