PRKAR1B Antibody

• Background:

  Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM].

• NCBI Gene ID:

  5575

• Swiss Prot:

  P31321

• Accession Number:

  P31321

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PRKAR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human PRKAR1B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  43 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PRKAR1B

• NCBI Official Name:

  CAMP-dependent protein kinase type I-beta regulatory subunit

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Silva, L.K., et al. Eur. J. Hum. Genet. (2010) In press :

• Background Reference 02:

  Liu, Y.J., et al. Obesity (Silver Spring) (2010) In press :

• Background Reference 03:

  Zhan, X., et al. Anal. Biochem. 354 (2) :279-289 (2006)

• Background Reference 04:

  Gullingsrud, J., et al. Structure 14 (1) :141-149 (2006)

• Other Product Names:

  CAMP-dependent protein kinase type I-beta regulatory subunit, PRKAR1B

• Tested Applications:

  WB

• Protein ID:

  229463042

• Physical Properties:

  Liquid