PRKAR2B Antibody

• Background:

  CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq].

• NCBI Gene ID:

  5577

• Swiss Prot:

  P31323

• Accession Number:

  P31323

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PRKAR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the Central region of human PRKAR2B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  46 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  PRKAR2B

• NCBI Official Name:

  CAMP-dependent protein kinase type II-beta regulatory subunit

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Liu, Y.J., et al. Obesity (Silver Spring) (2010) In press :

• Background Reference 02:

  Adkins, D.E., et al. Mol. Psychiatry (2010) In press :

• Background Reference 03:

  Islam, A., et al. J. Biol. Chem. 283 (37) :25364-25371 (2008)

• Background Reference 04:

  Ji, Z., et al. J. Biol. Chem. 283 (32) :21920-21925 (2008)

• Other Product Names:

  CAMP-dependent protein kinase type II-beta regulatory subunit, PRKAR2B

• Tested Applications:

  WB

• Protein ID:

  206729918

• Physical Properties:

  Liquid