DNMT3L Antibody

• Background:

  CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined.

• NCBI Gene ID:

  29947

• Swiss Prot:

  Q9UJW3

• Accession Number:

  Q9UJW3

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This DNMT3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-381 amino acids from the C-terminal region of human DNMT3L.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  44 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  DNMT3L

• NCBI Official Name:

  DNA (cytosine-5) -methyltransferase 3-like

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Holz-Schietinger, C., et al. J. Biol. Chem. 285 (38) :29091-29100 (2010)

• Background Reference 02:

  Manderwad, G.P., et al. Arch. Pathol. Lab. Med. 134 (8) :1193-1196 (2010)

• Background Reference 03:

  Kim, H., et al. Int. J. Oncol. 36 (6) :1563-1572 (2010)

• Background Reference 04:

  Minami, K., et al. Clin. Cancer Res. 16 (10) :2751-2759 (2010)

• Other Product Names:

  DNA (cytosine-5) -methyltransferase 3-like, DNMT3L

• Tested Applications:

  WB

• Protein ID:

  334302913

• Physical Properties:

  Liquid