DNMT3A Antibody

• Background:

  CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

• NCBI Gene ID:

  1788

• Swiss Prot:

  Q9Y6K1

• Accession Number:

  Q9Y6K1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This DNMT3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 872-900 amino acids from the C-terminal region of human DNMT3A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  102 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  DNMT3A

• NCBI Official Name:

  DNA (cytosine-5) -methyltransferase 3A

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Holz-Schietinger, C., et al. J. Biol. Chem. 285 (38) :29091-29100 (2010)

• Background Reference 02:

  Kelemen, L.E., et al. Cancer Epidemiol. Biomarkers Prev. 19 (7) :1822-1830 (2010)

• Background Reference 03:

  Park, C.W., et al.Cardiovasc Transl Res 3 (3) :290-295 (2010)

• Background Reference 04:

  Haggarty, P., et al. PLoS ONE (6), E11329 (2010) :

• Other Product Names:

  DNA (cytosine-5) -methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A

• Tested Applications:

  WB

• Protein ID:

  166215081

• Physical Properties:

  Liquid