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DPH1 Antibody

CAT: 0223-58-570Size: 400 µLDry Ice: NoHazardous: No
CAT#:0223-58-570Size:400 µL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610) . This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH1 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]) .[supplied by OMIM].
NCBI Gene ID
1801
Swiss Prot
Q9BZG8
Accession Number
Q9BZG8
Host
Rabbit
Reactivity
Human
Immunogen
This DPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 384-412 amino acids from the C-terminal region of human DPH1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
49 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
DPH1
NCBI Official Name
Diphthamide biosynthesis protein 1
NCBI Organism
Homo sapiens
Background Reference 01
Liu, S., et al. Mol. Cell. Biol. 24 (21) :9487-9497 (2004)
Background Reference 02
Cardoso, C., et al. Am. J. Hum. Genet. 72 (4) :918-930 (2003)
Background Reference 03
Chen, C.M., et al. Biochem. Biophys. Res. Commun. 286 (5) :1019-1026 (2001)
Background Reference 04
Salicioni, A.M., et al. Genomics 69 (1) :54-62 (2000)
Other Product Names
Diphthamide biosynthesis protein 1, DPH1 homolog, HsDph1, Diphthamide biosynthesis protein 2 homolog-like 1, DPH-like 1, DPH2-like 1, Diphthamide biosynthesis protein 2-like, Ovarian cancer-associated gene 1 protein, DPH1, DPH2L, DPH2L1, OVCA1
Tested Applications
WB
Protein ID
269849559
Physical Properties
Liquid

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