DPH2 Antibody

• Background:

  This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.

• NCBI Gene ID:

  1802

• Swiss Prot:

  Q9BQC3

• Accession Number:

  NP_001375

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DPH2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  52 kDa

• Applications Notes:

  DPH2 antibody can be used for detection of DPH2 by ELISA at 1:1562500. DPH2 antibody can be used for detection of DPH2 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  DPH2

• NCBI Official Name:

  DPH2 homolog (S. cerevisiae)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497.

• Other Product Names:

  DPH2, DPH2L2

• Tested Applications:

  ELISA, WB

• Protein ID:

  21536476

• Physical Properties:

  Liquid