DPH4 Antibody

CAT: 0223-30-874Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-30-874Size:100 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610) . This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2.Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610) . This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]) .
NCBI Gene ID
120526
Swiss Prot
Q6P3W2
Accession Number
NP_859057
Host
Rabbit
Reactivity
Human
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH4.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DPH4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
17 kDa
Applications Notes
DPH4 antibody can be used for detection of DPH4 by ELISA at 1:312500. DPH4 antibody can be used for detection of DPH4 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
DNAJC24
NCBI Official Name
DnaJ (Hsp40) homolog, subfamily C, member 24
NCBI Organism
Homo sapiens
Background Reference 01
Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497.
Other Product Names
DPH4, JJJ3, ZCSL3, DPH4
Tested Applications
ELISA, WB
Protein ID
89111951
Physical Properties
Liquid

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