DPH2 Antibody

CAT: 0223-59-759Size: 400 µLDry Ice: NoHazardous: No
CAT#:0223-59-759Size:400 µL
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Background
This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.
NCBI Gene ID
1802
Swiss Prot
Q9BQC3
Accession Number
Q9BQC3
Host
Rabbit
Reactivity
Human
Immunogen
This DPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-148 amino acids from the N-terminal region of human DPH2.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
52 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
DPH2
NCBI Official Name
Diphthamide biosynthesis protein 2
NCBI Organism
Homo sapiens
Background Reference 01
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 02
Liu, S., et al. Mol. Cell. Biol. 24 (21) :9487-9497 (2004)
Background Reference 03
Schultz, D.C., et al. Genomics 52 (2) :186-191 (1998)
Background Reference 04
Foley, B.T., et al. J. Biol. Chem. 270 (39) :23218-23225 (1995)
Other Product Names
Diphthamide biosynthesis protein 2, DPH2 homolog, HsDph2, Diphthamide biosynthesis protein 2 homolog-like 2, DPH2-like 2, DPH2, DPH2L2
Tested Applications
WB
Protein ID
74761201
Physical Properties
Liquid

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