MRPS15 Antibody

• Background:

  Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.

• NCBI Gene ID:

  64960

• Swiss Prot:

  P82914

• Accession Number:

  P82914

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MRPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-153 amino acids from the Central region of human MRPS15.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  30 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MRPS15

• NCBI Official Name:

  28S ribosomal protein S15, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhang, Z., et al. Genomics 81 (5) :468-480 (2003)

• Background Reference 02:

  Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :

• Background Reference 03:

  Suzuki, T., et al. J. Biol. Chem. 276 (35) :33181-33195 (2001)

• Background Reference 04:

  Cavdar Koc, E., et al. J. Biol. Chem. 276 (22) :19363-19374 (2001)

• Other Product Names:

  28S ribosomal protein S15, mitochondrial, MRP-S15, S15mt, MRPS15, RPMS15

• Tested Applications:

  WB

• Protein ID:

  13633907

• Physical Properties:

  Liquid