NEDD4L Antibody

• Background:

  E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK.

• NCBI Gene ID:

  23327

• Swiss Prot:

  Q96PU5

• Accession Number:

  Q96PU5

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This NEDD4L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-604 amino acids from the Central region of human NEDD4L.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  112 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NEDD4L

• NCBI Official Name:

  E3 ubiquitin-protein ligase NEDD4-like

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zhou, R., et al. J. Biol. Chem. 285 (40) :30523-30530 (2010)

• Background Reference 02:

  Raikwar, N.S., et al. Am. J. Physiol. Renal Physiol. 299 (2), F436-F444 (2010) :

• Background Reference 03:

  Hallows, K.R., et al. J. Biol. Chem. 285 (28) :21671-21678 (2010)

• Background Reference 04:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Other Product Names:

  E3 ubiquitin-protein ligase NEDD4-like, 632-, NEDD42, Nedd4-2, NEDD4L, KIAA0439, NEDL3

• Tested Applications:

  WB

• Protein ID:

  73921204

• Physical Properties:

  Liquid