ZAP70 Antibody

• Background:

  This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

• NCBI Gene ID:

  7535

• Swiss Prot:

  P43403

• Accession Number:

  P43403

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human ZAP70.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  70 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  ZAP70

• NCBI Official Name:

  Tyrosine-protein kinase ZAP-70

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Zanotti, R., et al. Am. J. Hematol. 85 (7) :494-498 (2010)

• Background Reference 02:

  Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010) :

• Background Reference 03:

  Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (22) :10166-10171 (2010)

• Background Reference 04:

  Kotaskova, J., et al.Mol Diagn 12 (3) :328-334 (2010)

• Other Product Names:

  Tyrosine-protein kinase ZAP-70, 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, ZAP70, SRK

• Tested Applications:

  WB, IHC-P, Flow

• Protein ID:

  1177044

• Physical Properties:

  Liquid