H2AFX Antibody

• Background:

  Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

• NCBI Gene ID:

  3014

• Swiss Prot:

  P16104

• Accession Number:

  P16104

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This H2AFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human H2AFX.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  15 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  H2AFX

• NCBI Official Name:

  Histone H2AX

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Roch-Lefevre, S., et al. Radiat. Res. 174 (2) :185-194 (2010)

• Background Reference 02:

  Schmid, T.E., et al. Int. J. Radiat. Biol. 86 (8) :682-691 (2010)

• Background Reference 03:

  Jiang, X., et al. FEBS Lett. 584 (13) :2926-2930 (2010)

• Background Reference 04:

  Vasireddy, R.S., et al. Br. J. Cancer 102 (10) :1511-1518 (2010)

• Other Product Names:

  Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX

• Tested Applications:

  WB, IHC-P

• Protein ID:

  121992

• Physical Properties:

  Liquid