GITRL Antibody

• Background:

  GITRL is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e. g. testis, .) . GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T-cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.

• NCBI Gene ID:

  8995

• Swiss Prot:

  Q9UNG2

• Accession Number:

  13124621

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human GITRL.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Immunology

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity:

  Recognizes human GITRL. Detects a band of ~15-17kDa by Western blot. Does not cross-react with mouse GITRL.

• Applications Notes:

  ELISA: (direct and indirect: 1:5,000-1:10,000) . Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol:

  TNFSF18

• NCBI Official Name:

  Tumor necrosis factor (ligand) superfamily, member 18

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Glucocorticoid-induced TNF Receptor Ligand; AITRL; Activation-inducible TNF-related Ligand; TNFSF18

• Tested Applications:

  ELISA, WB

• Protein ID:

  Q9UNG2

• Physical Properties:

  Liquid