PPAT Antibody

• Background :

  PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. Its gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.

• NCBI Gene ID :

  5471

• Swiss Prot :

  Q06203

• Accession Number :

  NP_002694

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPAT.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Immunology

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PPAT antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  56 kDa

• Applications Notes :

  PPAT antibody can be used for detection of PPAT by ELISA at 1:62500. PPAT antibody can be used for detection of PPAT by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  PPAT

• NCBI Official Name :

  Phosphoribosyl pyrophosphate amidotransferase

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Bera, A.K., (1999) J. Biol. Chem. 274 (51), 36498-36504.

• Other Product Names :

  PPAT, ATASE, GPAT, PRAT

• Tested Applications :

  ELISA, WB, IHC

• Protein ID :

  29570798

• Physical Properties :

  Liquid