PPAT Antibody

• Background:

  PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. Its gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.

• NCBI Gene ID:

  5471

• Swiss Prot:

  Q06203

• Accession Number:

  NP_002694

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPAT.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PPAT antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  56 kDa

• Applications Notes:

  PPAT antibody can be used for detection of PPAT by ELISA at 1:62500. PPAT antibody can be used for detection of PPAT by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PPAT

• NCBI Official Name:

  Phosphoribosyl pyrophosphate amidotransferase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Bera, A.K., (1999) J. Biol. Chem. 274 (51), 36498-36504.

• Other Product Names:

  PPAT, ATASE, GPAT, PRAT

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  29570798

• Physical Properties:

  Liquid