EIF2S3 Antibody

• Background:

  EIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

• NCBI Gene ID:

  1968

• Swiss Prot:

  P41091

• Accession Number:

  NP_001406

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF2S3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  51 kDa

• Applications Notes:

  EIF2S3 antibody can be used for detection of EIF2S3 by ELISA at 1:312500. EIF2S3 antibody can be used for detection of EIF2S3 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  EIF2S3

• NCBI Official Name:

  Eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gevaert, K., (2003) Nat. Biotechnol. 21 (5), 566-569.

• Other Product Names:

  EIF2S3, EIF2, EIF2G, EIF2gamma, eIF-2gA

• Tested Applications:

  ELISA, WB

• Protein ID:

  4503507

• Physical Properties:

  Liquid