EIF2S1 Antibody

• Background:

  The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD), and gamma (52 kD) . The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha.The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987) .

• NCBI Gene ID:

  1965

• Swiss Prot:

  P05198

• Accession Number:

  NP_004085

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF2S1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  35 kDa

• Applications Notes:

  EIF2S1 antibody can be used for detection of EIF2S1 by ELISA at 1:2500. EIF2S1 antibody can be used for detection of EIF2S1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  EIF2S1

• NCBI Official Name:

  Eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Marciniak, S.J., (2006) J. Cell Biol. 172 (2), 201-209.

• Other Product Names:

  EIF2S1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  4758256

• Physical Properties:

  Liquid