EIF2S1 Antibody

CAT: 0223-29-326Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-29-326Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD), and gamma (52 kD) . The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha.The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987) .
NCBI Gene ID
1965
Swiss Prot
P05198
Accession Number
NP_004085
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Zebrafish
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF2S1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
35 kDa
Applications Notes
EIF2S1 antibody can be used for detection of EIF2S1 by ELISA at 1:2500. EIF2S1 antibody can be used for detection of EIF2S1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
EIF2S1
NCBI Official Name
Eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
NCBI Organism
Homo sapiens
Background Reference 01
Marciniak, S.J., (2006) J. Cell Biol. 172 (2), 201-209.
Other Product Names
EIF2S1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A
Tested Applications
ELISA, WB, IHC
Protein ID
4758256
Physical Properties
Liquid

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