EIF2S1 Antibody

• Background :

  The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD), and gamma (52 kD) . The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha.The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987) .

• NCBI Gene ID :

  1965

• Swiss Prot :

  P05198

• Accession Number :

  NP_004085

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S1.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store EIF2S1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  35 kDa

• Applications Notes :

  EIF2S1 antibody can be used for detection of EIF2S1 by ELISA at 1:2500. EIF2S1 antibody can be used for detection of EIF2S1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  EIF2S1

• NCBI Official Name :

  Eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Marciniak, S.J., (2006) J. Cell Biol. 172 (2), 201-209.

• Other Product Names :

  EIF2S1, EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A

• Tested Applications :

  ELISA, WB, IHC

• Protein ID :

  4758256

• Physical Properties :

  Liquid