PPAP2A Antibody

• Background :

  PPAP2A is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of PPAP2A is found to be regulated by androgen in a prostatic adenocarcinoma cell line.The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of this gene is found to be regulated by androgen in a prostatic adenocarcinoma cell line. At least two alternatively spliced transcript variants encoding distinct isoforms have been described.

• NCBI Gene ID :

  8611

• Swiss Prot :

  O14494-2

• Accession Number :

  NP_795714

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Zebrafish

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPAP2A.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PPAP2A antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  31 kDa, 32 kDa

• Applications Notes :

  PPAP2A antibody can be used for detection of PPAP2A by ELISA at 1:62500. PPAP2A antibody can be used for detection of PPAP2A by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  PPAP2A

• NCBI Official Name :

  Phosphatidic acid phosphatase type 2A

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Tanyi, J.L., Clin. Cancer Res. 9 (10 PT 1), 3534-3545 (2003) .

• Other Product Names :

  PPAP2A, LLP1a, LPP1, PAP-2a, PAP2, PAP2a2, PAP2alpha2, PAPalpha1

• Tested Applications :

  ELISA, WB

• Protein ID :

  29171738

• Physical Properties :

  Liquid