PPARA Antibody

• Background:

  Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals which contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor.

• NCBI Gene ID:

  5465

• Swiss Prot:

  Q07869

• Accession Number:

  NP_005027

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPARA.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer, Signal Transduction

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PPARA antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  51 kDa

• Applications Notes:

  PPARA antibody can be used for detection of PPARA by ELISA at 1:12500. PPARA antibody can be used for detection of PPARA by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PPARA

• NCBI Official Name:

  Peroxisome proliferator-activated receptor alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  S, et al., (2004) Gastroenterology 127 (5), 1436-1445.

• Other Product Names:

  PPARA, PPAR, NR1C1, hPPAR, PPARalpha

• Tested Applications:

  ELISA, WB

• Protein ID:

  7549811

• Physical Properties:

  Liquid