ACO1 Antibody

• Background:

  ACO1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs) . It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs) . IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.

• NCBI Gene ID:

  48

• Swiss Prot:

  P28271

• Accession Number:

  NP_002188

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ACO1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. XBL-10408 - Fetal Kidney Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ACO1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  98 kDa

• Applications Notes:

  ACO1 antibody can be used for detection of ACO1 by ELISA at 1:62500. ACO1 antibody can be used for detection of ACO1 by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ACO1

• NCBI Official Name:

  Aconitase 1, soluble

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Clarke, S.L., (2006) EMBO J. 25 (3), 544-553.

• Other Product Names:

  ACO1, IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  8659555

• Physical Properties:

  Liquid