NR5A2 Antibody

• Background:

  NR5A2 binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. It may be responsable for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. It is a key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. It may also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.

• NCBI Gene ID:

  2494

• Swiss Prot:

  O00482

• Accession Number:

  NP_995582

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR5A2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NR5A2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  61 kDa, 56 kDa

• Applications Notes:

  NR5A2 antibody can be used for detection of NR5A2 by ELISA at 1:12500. NR5A2 antibody can be used for detection of NR5A2 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NR5A2

• NCBI Official Name:

  Nuclear receptor subfamily 5, group A, member 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lee, Y.K., (2006) J. Biol. Chem. 281 (12), 7850-7855.

• Other Product Names:

  NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, hB1F, hB1F-2, LRH1

• Tested Applications:

  ELISA, WB

• Protein ID:

  45545405

• Physical Properties:

  Liquid