MCM3 Antibody

MCM3 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

4172

P25205

NP_002379

Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM3.

Unconjugated

Primary Antibodies

Cell Cycle, Cancer

Antibody is purified by peptide affinity chromatography method.

Cat. No. 1205 - Jurkat Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MCM3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

89 kDa

MCM3 antibody can be used for detection of MCM3 by ELISA at 1:12500. MCM3 antibody can be used for detection of MCM3 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

MCM3

Minichromosome maintenance complex component 3

Homo sapiens

Shukla, A., (2005) Biochem. Biophys. Res. Commun. 329 (4), 1240-1245.

MCM3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB

ELISA, WB, IHC

394582093