MCM4 Antibody

• Background:

  The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

• NCBI Gene ID:

  4173

• Swiss Prot:

  P33991

• Accession Number:

  NP_877423

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MCM4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  95 kDa, 97 kDa

• Applications Notes:

  MCM4 antibody can be used for detection of MCM4 by ELISA at 1:312500. MCM4 antibody can be used for detection of MCM4 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MCM4

• NCBI Official Name:

  Minichromosome maintenance complex component 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ishimi, Y., et al., (2003) J. Biol. Chem. 278 (27), 24644-24650.

• Other Product Names:

  MCM4, NKCD, CDC21, CDC54, NKGCD, hCdc21, P1-CDC21

• Tested Applications:

  ELISA, WB

• Protein ID:

  33469917

• Physical Properties:

  Liquid