MCM7 Antibody

• Background:

  The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

• NCBI Gene ID:

  4176

• Swiss Prot:

  P33993

• Accession Number:

  P33993

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MCM7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the N-terminal region of human MCM7.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  81 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MCM7

• NCBI Official Name:

  DNA replication licensing factor MCM7

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lau, K.M., et al. Oncogene 29 (40) :5475-5489 (2010)

• Background Reference 02:

  Kim, D.W., et al. Mol. Biochem. Parasitol. 173 (1) :10-16 (2010)

• Background Reference 03:

  Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :

• Background Reference 04:

  Rojiani, M.V., et al. Appl. Immunohistochem. Mol. Morphol. 18 (3) :278-282 (2010)

• Other Product Names:

  DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2

• Tested Applications:

  WB

• Protein ID:

  20981696

• Physical Properties:

  Liquid