SPIB Antibody

• Background:

  SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime) .

• NCBI Gene ID:

  6689

• Swiss Prot:

  Q01892

• Accession Number:

  NP_003112

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPIB.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1224 - Daudi Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store SPIB antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  19 kDa

• Applications Notes:

  SPIB antibody can be used for detection of SPIB by ELISA at 1:62500. SPIB antibody can be used for detection of SPIB by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  SPIB

• NCBI Official Name:

  Spi-B transcription factor (Spi-1/PU.1 related)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Geng, C.D. (2005) J. Biol. Chem. 280 (52), 43264-43271.

• Other Product Names:

  SPIB, SPI-B

• Tested Applications:

  ELISA, WB

• Protein ID:

  61888836

• Physical Properties:

  Liquid