MAP3K8 Antibody

• Background:

  MAP3K8 is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1, p105 (NFKB1) . This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity.

• NCBI Gene ID:

  1326

• Swiss Prot:

  P41279

• Accession Number:

  NP_005195

• Host:

  Rabbit

• Reactivity:

  Human, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAP3K8.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MAP3K8 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  53 kDa

• Applications Notes:

  MAP3K8 antibody can be used for detection of MAP3K8 by ELISA at 1:1562500. MAP3K8 antibody can be used for detection of MAP3K8 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MAP3K8

• NCBI Official Name:

  Mitogen-activated protein kinase kinase kinase 8

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jia, Y., et al., (2005) Arch. Biochem. Biophys. 441 (1), 64-74.

• Other Product Names:

  MAP3K8, COT, EST, ESTF, TPL2, MEKK8, Tpl-2, c-COT

• Tested Applications:

  ELISA, WB

• Protein ID:

  22035598

• Physical Properties:

  Liquid