MARCH2 Antibody

• Background:

  MARCH2 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH2 may be involved in endosomal trafficking through interaction with STX6.

• NCBI Gene ID:

  51257

• Swiss Prot:

  Q9P0N8

• Accession Number:

  NP_001005415

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MARCH2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MARCH2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  27 kDa

• Applications Notes:

  MARCH2 antibody can be used for detection of 40239 by ELISA at 1:312500. MARCH2 antibody can be used for detection of 40239 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MARCH2

• NCBI Official Name:

  Membrane-associated ring finger (C3HC4) 2, E3 ubiquitin protein ligase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cao, Z., (2008) Cell. Signal. 20 (1), 73-82.

• Other Product Names:

  MARCH2, HSPC240, MARCH-II, RNF172

• Tested Applications:

  ELISA, WB

• Protein ID:

  53729332

• Physical Properties:

  Liquid