MARCH9 Antibody

• Background:

  MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19) . MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Hoer et al., 2007 [PubMed 17174307]) .[supplied by OMIM].

• NCBI Gene ID:

  92979

• Swiss Prot:

  Q86YJ5

• Accession Number:

  Q86YJ5

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MARCH9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the C-terminal region of human MARCH9.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  38 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MARCH2

• NCBI Official Name:

  E3 ubiquitin-protein ligase MARCH9

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nice, T.J., et al. J. Immunol. 185 (9) :5369-5376 (2010)

• Background Reference 02:

  Bailey, S.D., et al. Diabetes Care 33 (10) :2250-2253 (2010)

• Background Reference 03:

  Talmud, P.J., et al. Am. J. Hum. Genet. 85 (5) :628-642 (2009)

• Background Reference 04:

  Hoer, S., et al. FEBS Lett. 581 (1) :45-51 (2007)

• Other Product Names:

  E3 ubiquitin-protein ligase MARCH9, 632-, Membrane-associated RING finger protein 9, Membrane-associated RING-CH protein IX, MARCH-IX, RING finger protein 179, MARCH9, RNF179

• Tested Applications:

  WB

• Protein ID:

  74759533

• Physical Properties:

  Liquid