MARCH7 Antibody

• Background:

  MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19) . MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]) .

• NCBI Gene ID:

  64844

• Swiss Prot:

  Q9H992

• Accession Number:

  Q9H992

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This MARCH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the N-terminal region of human MARCH7.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  78 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  MARCH7

• NCBI Official Name:

  E3 ubiquitin-protein ligase MARCH7

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Szigyarto, C.A., et al. J. Histochem. Cytochem. 58 (4) :301-308 (2010)

• Background Reference 02:

  Nathan, J.A., et al. Traffic 9 (7) :1130-1145 (2008)

• Background Reference 03:

  Muthukumarana, P.A., et al. Int. Immunopharmacol. (13-14), 1993-2001 (2006) :

• Background Reference 04:

  Flierman, D., et al. Proc. Natl. Acad. Sci. U.S.A. 103 (31) :11589-11594 (2006)

• Other Product Names:

  E3 ubiquitin-protein ligase MARCH7, 632-, Axotrophin, Membrane-associated RING finger protein 7, Membrane-associated RING-CH protein VII, MARCH-VII, RING finger protein 177, MARCH7, AXOT, RNF177

• Tested Applications:

  WB

• Protein ID:

  74762745

• Physical Properties:

  Liquid