CRYBB2 Antibody

• Background:

  Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group) . Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, is part of a gene cluster with beta-A4, beta-B1, and beta-B3. A chain-terminating mutation was found to cause type 2 cerulean cataracts.

• NCBI Gene ID:

  1415

• Swiss Prot:

  P43320

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human CRYBB2 (NP_000487.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Affinity purification

• Positive Control:

  THP-1

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 27kDa

• Fragment:

  IgG

• Applications Notes:

  WB: 1:500 - 1:2000

• Symbol:

  CRYBB2

• Positive Control 2:

  SW620

• Positive Control 3:

  HeLa

• Positive Control 4:

  Mouse eye

• Positive Control 5:

  Rat eye

• NCBI Official Name:

  Crystallin beta B2

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CCA2, CRYB2, CRYB2A, CTRCT3, D22S665, beta-crystallin B2, CTA-221G9.7, beta-B2 crystallin, beta-crystallin Bp, eye lens structural protein

• Tested Applications:

  WB

• Physical Properties:

  Liquid