RAD17 Antibody

• Background:

  The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

• NCBI Gene ID:

  5884

• Swiss Prot:

  O75943

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1) .

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Affinity purification

• Positive Control:

  SW480

• Concentration:

  Batch dependent

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at -20˚ C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  Observed: 77kDa

• Fragment:

  IgG

• Symbol:

  RAD17

• Positive Control 2:

  BT-474

• Positive Control 3:

  K-562

• Positive Control 4:

  22Rv1

• Positive Control 5:

  Raji

• NCBI Official Name:

  RAD17 homolog (S. pombe)

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  RAD17, CCYC, HRAD17, R24L, RAD17Sp, Rad24, RAD24, RAD17SP

• Tested Applications:

  WB, IF, IP

• Physical Properties:

  Liquid