PAK4 Antibody

• Background:

  PAK4 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

• NCBI Gene ID:

  10298

• Swiss Prot:

  O96013

• Accession Number:

  NP_005875

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction, Neuroscience, Cancer

• Purification:

  PAK4 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1221 - SW480 Cell Lysate

• Concentration:

  1 mg/mL

• Buffer:

  PAK4 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  PAK4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Specificity:

  PAK4 antibody is predicted to not cross-react with other PAK family proteins.

• Symbol:

  PAK4

• Positive Control 2:

  Cat. No. 1320 - Human Colon Tissue Lysate

• NCBI Official Name:

  P21 protein (Cdc42/Rac) -activated kinase 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7.

• Background Reference 02:

  Abo A, Qu J, Cammarano MS, et al. PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia. EMBO J. 1998; 17:6527-40.

• Background Reference 03:

  Cotteret S, Jaffer ZM, Beeser A, et al. p21-activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylated BAD. Mol. Cell. Biol. 2003; 23:5526-39.

• Other Product Names:

  PAK4 Antibody: KIAA1142, p21-activated kinase 4, PAK-4

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  5031975

• Physical Properties:

  Liquid