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PAK2 Antibody

CAT: 0223-3887-01Size: 0.02 mgDry Ice: NoHazardous: No
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CAT#:0223-3887-01Size:0.02 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PAK2 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.
NCBI Gene ID
5062
Swiss Prot
Q13177
Accession Number
NP_002568
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
3887
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar PAK2 expression profile in human, mouse and rat cell lines detected by two independent anti-PAK2 antibodies that recognize different epitopes, 3885 against C-terminus domain and 3887 against N-terminus domain. PAK2 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3885 3887
Field of Research
Signal Transduction, Apoptosis, Cancer
Purification
PAK2 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Rabbit: (93%)
Buffer
PAK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
PAK2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human PAK2 has only one isoform (524aa, 58kD) . Mouse PAK2 has one isoform (524aa, 58kD) and rat PAK2 also has one isoform (524aa, 58kD) . 3887 can detect the isoforms of human, mouse and rat.
Symbol
PAK2
Positive Control 2
Cat. No. 1406 - Mouse Spleen Tissue Lysate
NCBI Official Name
P21 protein (Cdc42/Rac) -activated kinase 2
NCBI Organism
Homo sapiens
Background Reference 01
Jaffer and Chernoff. Int. J. Biochem. Cell Biol. 2002; 34:713-7.
Background Reference 02
Rudel and Bokoch. Science 1997; 276:1571-4.
Background Reference 03
Vilas, et al. Proc. Natl. Acad. Sci. USA 2006; 103:6542-7.
Other Product Names
PAK2 Antibody: PAK65, PAKgammaGamma-PAK, PAK-2
Tested Applications
ELISA, WB, IHC-P, IF
Protein ID
32483399
Physical Properties
Liquid

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