PAK5 Antibody

• Background :

  PAK5 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK5 was initially identified as a kinase expressed primarily in brain that while possessing a kinase domain and GTPase binding domain similar to PAK4 and PAK6, is completely different from both. Expression of PAK5 in neural based cell lines resulted in neurite outgrowth suggesting that PAK5 may be involved in regulating the cytoskeletal changes necessary for promoting neurite outgrowth. Other experiments suggest that unlike the other PAKs, PAK5 may inhibit apoptosis by phosphorylating the Bcl-2 family member Bad.

• NCBI Gene ID :

  57144

• Swiss Prot :

  Q9P286

• Accession Number :

  Q9P286

• Host :

  Rabbit

• Reactivity :

  Human

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Signal Transduction, Homeostasis, Neuroscience

• Purification :

  PAK5 Antibody is affinity chromatography purified via peptide column.

• Positive Control :

  Cat. No. 1213 - T24 Cell Lysate

• Concentration :

  1 mg/mL

• Homology :

  Predicted species reactivity based on immunogen sequence: Mouse: (100%), Rat: (92%)

• Buffer :

  PAK5 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  PAK5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment :

  IgG

• Symbol :

  PAK7

• Positive Control 2 :

  Cat. No. 1204 - K562 Cell Lysate

• Positive Control 3 :

  Cat. No. 17-004 - K-562 Cell Slide

• NCBI Official Name :

  P21 protein (Cdc42/Rac) -activated kinase 7

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7.

• Background Reference 02 :

  Dan C, Nath N, Liberto M, et al. PAK5, a new brain-specific kinase, promotes neurite outgrowth in N1E-115 cells. Mol. Cell. Biol. 2002; 22:567-77.

• Background Reference 03 :

  Cotteret S, Jaffer ZM, Beeser A, et al. p21-activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylated BAD. Mol. Cell. Biol. 2003; 23:5526-39.

• Other Product Names :

  PAK5 Antibody: PAK5, KIAA1264, PAK5, p21-activated kinase 5, PAK-5

• Tested Applications :

  ELISA, WB, ICC, IF

• Protein ID :

  12585290

• Physical Properties :

  Liquid