Srf (NM_020493) Mouse Recombinant Protein

• Background:

  SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (PubMed:24732378) . Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12732141, PubMed:19350017, PubMed:24732378) . The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378) . Required for cardiac differentiation and maturation (PubMed:15169892) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AW049942; AW240594

• Gene ID:

  20807

• UniProt:

  Q9JM73

• Accession Number mRNA:

  NM_020493

• Chromosomal Location:

  17 C

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  51.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Srf

• Species:

  Mouse

• Protein ID:

  NP_065239

• Overview:

  Purified recombinant protein of Mouse serum response factor (Srf), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/20807

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9JM73

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_020493

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_065239