LIF Protein, Mouse, Recombinant

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins.

A DNA sequence encoding the mouse LIF (NP_032527.1) (Met1-Phe203) was expressed and purified.

Leukemia inhibitory factor

HEK293 Cells

No tag

< 1.0 EU per μg of the protein as determined by the LAL method.

> 95 % as determined by SDS-PAGE.

Measured by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells.The EDfor this effect is typically 0.1-0.5 ng/mL.

Lyophilized

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Cell Therapy proteins, Biological Target proteins

The recombinant mouse LIF consists of 180 amino acids and predicts a molecular mass of 19.9 kDa. It migrates as approximately 34.1 kDa band in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

LIF

Mouse

NP_032527.1

Met1-Phe203