S100A4 Protein, Mouse, Recombinant

• Background:

  S100A4, also known as metastasis-associated protein Mtsl, belongs to the family of small calcium-binding S100 proteins containing two EF-hand calcium-binding motifs. In humans at least 20 S100 family members that are distributed tissue specifically have been identified, and are involved in a number of cellular processes as transducers of calcium signal. S100A4 is a symmetric homodimer, and undergoes a relatively large conformational change upon the typical EF-hand binding calcium, which is necessary for S100A4 to interact with its protein targets and generate biological effects. It can bind the already known targets p53, F-actin, liprin β, myosin heavy chain II, and prevent their phosphorylation and multimerization. It has been demonstrated that S100A4 is directly involved in tumor metastasis including cell motility, invasion, apoptosis, angiogenesis and differentiation, and appears to be a metastasis factor and a molecular marker for clinical prognosis. Multiple alternatively spliced variants encoding the same protein have been identified.

• Synonyms:

  18A2 Protein, Mouse; 42a Protein, Mouse; Capl Protein, Mouse; FSp1 Protein, Mouse; metastasin Protein, Mouse; Mts1 Protein, Mouse; PeL98 Protein, Mouse; pk9a Protein, Mouse

• Gene Name:

  S100 calcium binding protein A4

• UniProt:

  P07091

• Expression System:

  HEK293 Cells

• Tag:

  No tag

• Field of Research:

  Cancer Drug Targets Calcium-binding Proteins and Related Molecules

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biomarker Proteins, Diagnostic proteins

• Molecular Weight:

  The recombinant mouse S100A4 consists of 102 amino acids and predicts a molecular mass of 11.7 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  S100A4

• Species:

  Mouse

• Overview:

  A DNA sequence encoding the mouse S100A4 (P07091) (Ala2-Lys101) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P07091

• AA Sequence:

  Ala2-Lys101