UBE2B Protein, Mouse, Recombinant

• Background:

  The ubiquitin-conjugating enzyme E2B (Ube2b) is a critical target gene of androgen receptor (AR), mediating the function of AR in spermatogenesis by promoting H2A ubiquitylation. Moreover, UBE2B plays an important role in muscle protein homeostasis during catabolic conditions.

• Synonyms:

  2610301N02Rik Protein, Mouse; E2-14k Protein, Mouse; HR6B Protein, Mouse; mHR6B Protein, Mouse; Rad6b Protein, Mouse

• Gene Name:

  Ubiquitin-conjugating enzyme E2B

• Expression System:

  E. coli

• Tag:

  No tag

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20 mM Tris, 500 mM Nacl, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant mouse UBE2B consists 152 amino acids and predicts a molecular mass of 17.3 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  UBE2B

• Species:

  Mouse

• Protein ID:

  NP_003328.1

• Overview:

  A DNA sequence encoding the mouse UBE2B (NP_003328.1) (Met1-Ser152) was expressed.

• AA Sequence:

  Met1-Ser152

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_003328.1