IL3 Protein, Mouse, Recombinant

• Background:

  IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

• Synonyms:

  BPA Protein, Mouse; Csfmu Protein, Mouse; HCGF Protein, Mouse; Il-3 Protein, Mouse; MCGF Protein, Mouse; PSF Protein, Mouse

• Gene Name:

  Interleukin 3

• Expression System:

  HEK293 Cells

• Tag:

  No tag

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The EDfor this effect is 0.1-0.5 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Biological Target proteins, Cell Therapy proteins

• Molecular Weight:

  The recombinant mouse IL3 consists of 140 amino acids and predicts a molecular mass of 15.7 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL3

• Species:

  Mouse

• Protein ID:

  NP_034686.2

• Overview:

  A DNA sequence encoding the mouse IL3 (NP_034686.2) (Met1-Cys166) was expressed.

• AA Sequence:

  Met1-Cys166

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_034686.2