IdeS Protein, Streptococcus pyogenes, Recombinant (His Tag)

• Background:

  IdeS (IgG-degrading enzyme of Streptococcus pyogenes) is a secreted cysteine endopeptidase from the human pathogen S. pyogenes with an extraordinarily high degree of substrate specificity, catalyzing a single proteolytic cleavage at the lower hinge of human IgG. This proteolytic degradation promotes inhibition of opsonophagocytosis and interferes with the killing of group A Streptococcus.

• UniProt:

  F8V4V0-1

• Expression System:

  E. coli

• Tag:

  N-His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  One unit digests ≥ 95% of 1μg human IgG when incubated in PBS buffer, pH 7.4 at 37°C for 30min. The specific activity is 20,000 unit/mg.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, 50 % Glycerol, pH 6.6. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant streptococcus pyogenes IdeS consists of 319 amino acids and predicts a molecular mass of 36.2 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SD90_RS03345

• Species:

  Streptococcus pyogenes

• Overview:

  A DNA sequence encoding the streptococcus pyogenes IdeS (F8V4V0-1) (Asp30-Asn341) was expressed with a polyhistidine tag at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/F8V4V0-1

• AA Sequence:

  Asp30-Asn341