IdeS Protein, Streptococcus pyogenes, Recombinant (His & GST Tag)

• Background:

  IdeS (IgG-degrading enzyme of Streptococcus pyogenes) is a secreted cysteine endopeptidase from the human pathogen S. pyogenes with an extraordinarily high degree of substrate specificity, catalyzing a single proteolytic cleavage at the lower hinge of human IgG. This proteolytic degradation promotes inhibition of opsonophagocytosis and interferes with the killing of group A Streptococcus.

• UniProt:

  F8V4V0-1

• Expression System:

  E. coli

• Tag:

  N-GST & His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  One unit digests ≥ 95% of 1μg human IgG when incubated in PBS buffer, pH 7.4 at 37°C for 30min. The specific activity is 20,000 unit/mg.

• Form:

  Liquid

• Buffer:

  Supplied as sterile PBS, 50 % glycerol, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant streptococcus pyogenes IdeS consists of 549 amino acids and predicts a molecular mass of 63.1 kDa.

• Storage Conditions:

  Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  SD90_RS03345

• Species:

  Streptococcus pyogenes

• Overview:

  A DNA sequence encoding the streptococcus pyogenes IdeS (F8V4V0-1) (Asp30-Asn341) was expressed with the N-terminal polyhistidine-tagged a GST tag at the N-terminus (his-GST)

• Uniprot URL:

  https://www.uniprot.org/uniprot/F8V4V0-1

• AA Sequence:

  Asp30-Asn341