CRISPR-Cas9 Protein, Streptococcus pyogenes, Recombinant (His Tag)

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  1 ug = 5 U1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37°C

• Form:

  Liquid

• Buffer:

  Supplied from sterile 20 mM Tris, 300 mM NaCl, 50 % glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The recombinant s. pyogenes serotype M1 cas9 consists of 1407 amino acids and predicts a molecular mass of 163 kDa.

• Storage Conditions:

  Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CRISPR-Cas9

• Species:

  Streptococcus pyogenes

• Protein ID:

  WP_010922251.1

• Overview:

  A DNA sequence encoding the s. pyogenes serotype M1 cas9 (WP_010922251.1) (Met1-Asp1368) was expressed with a nuclear localization sequence (NLS) at the N-terminus, two NLSs and a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Asp1368

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/WP_010922251.1