CAS9 Streptococcus Pyogenes Recombinant Protein

• Expression System:

  E. coli

• Tag:

  N-His

• Concentration:

  20 uM

• Endotoxin:

  < 0.5 EU per 1 µg of the protein by the LAL

• Purity:

  > 95% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  In vitro digestion of a DNA fragment with the recombinant S.pyrogene Cas9 nuclease. The input DNA is XmnI-linearized vector was cleaved by with a synthetic sgRNA.

• Form:

  Liquid

• Buffer:

  10mM Tris, 300mM NaCl, 0.1mM EDTA, 1mM DTT, pH7.4, 50% glycerol.

• Molecular Weight:

  165.0 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CAS9

• Species:

  Streptococcus Pyogenes

• Overview:

  Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli