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CAS9 Streptococcus Pyogenes Recombinant Protein

CAT: 0882-PO49243E1Size: 500 pmolDry Ice: NoHazardous: No
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CAT#:0882-PO49243E1Size:500 pmol
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Overview
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
Expression System
E. coli
Tag
N-His
Concentration
20 uM
Endotoxin
< 0.5 EU per 1 µg of the protein by the LAL
Purity
> 95% as determined by SDS-PAGE and Coomassie blue staining
Activity
In vitro digestion of a DNA fragment with the recombinant S.pyrogene Cas9 nuclease. The input DNA is XmnI-linearized vector was cleaved by with a synthetic sgRNA.
Form
Liquid
Buffer
10mM Tris, 300mM NaCl, 0.1mM EDTA, 1mM DTT, pH7.4, 50% glycerol.
Molecular Weight
165.0 kDa
Storage Conditions
Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
CAS9
Species
Streptococcus Pyogenes

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