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Streptococcus pyogenes IdeS Recombinant Protein

CAT: 0223-11-129Size: 2500 UDry Ice: YesHazardous: No
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CAT#:0223-11-129Size:2500 U
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
IdeS from Streptococcus pyogenes, also known as Mac-1, is an endopeptidase with specificity for IgG. The bacterial protein Mac-1 adopts an alpha/beta fold, with 14 beta strands and 9 alpha helices. The N-terminal domain is made up predominantly of alpha helices, whereas the C-terminal domain consists predominantly of beta sheets. Mac 1 blocks polymorphonuclear opsonophagocytosis, inhibits the production of reactive oxygen species and contains IgG endopeptidase activity.IdeS is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F (ab‘) 2 and Fc/2 fragments. IdeS digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species. IdeS is cloned from Streptococcus pyogenes and expressed in E. coli.
CAS Number
9000-83-3
Accession Number
WP_010922160.1
Tag
His Tag
Type
Recombinant Proteins
Source
E. coli
Purity
>95% as determined by SDS-PAGE.
Buffer
PBS, pH7.4
Shipping Conditions
Dry Ice
Storage Conditions
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.
Calculated Molecular Weight
36.8 kDa
Applications Notes
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.8 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) .
Background Reference 01
Wenig K, Chatwell L, et al. Proc. Natl. Acad. Sci. U.S.A. 101, 17371-6, (2004) .
Background Reference 02
Hulting G, Flock M, et al. FEMS Microbiol. Lett. 298, 44-50, (2009) .
Background Reference 03
Seele J, Singpiel A, et al. J. Bacteriol. 195, 930-40, (2013) .
Other Product Names
IgG-degrading protease, Sib38, IdeS
Tested Applications
WB
Species
Streptococcus pyogenes
Physical Properties
Liquid

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