Streptococcus pyogenes IdeS Recombinant Protein

• Background:

  IdeS from Streptococcus pyogenes, also known as Mac-1, is an endopeptidase with specificity for IgG. The bacterial protein Mac-1 adopts an alpha/beta fold, with 14 beta strands and 9 alpha helices. The N-terminal domain is made up predominantly of alpha helices, whereas the C-terminal domain consists predominantly of beta sheets. Mac 1 blocks polymorphonuclear opsonophagocytosis, inhibits the production of reactive oxygen species and contains IgG endopeptidase activity.IdeS is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F (ab‘) 2 and Fc/2 fragments. IdeS digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species. IdeS is cloned from Streptococcus pyogenes and expressed in E. coli.

• Accession Number:

  WP_010922160.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Purity:

  >95% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  36.8 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.8 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) .

• Background Reference 01:

  Wenig K, Chatwell L, et al. Proc. Natl. Acad. Sci. U.S.A. 101, 17371-6, (2004) .

• Background Reference 02:

  Hulting G, Flock M, et al. FEMS Microbiol. Lett. 298, 44-50, (2009) .

• Background Reference 03:

  Seele J, Singpiel A, et al. J. Bacteriol. 195, 930-40, (2013) .

• Other Product Names:

  IgG-degrading protease, Sib38, IdeS

• Tested Applications:

  WB

• Species:

  Streptococcus pyogenes

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3