EasyStep Mouse APOB100 (Apolipoprotein B100) ELISA Kit

• Product Name Alternative:

  Apo-B100

• Reactivity:

  Mouse

• Field of Research:

  Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOB100, and the Mouse APOB100 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Mouse APOB100. After TMB substrate solution is added, only those wells that contain Mouse APOB100 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1.5h

• Standard:

  10 μg/mL

• Sample Type:

  Serum, plasma

• Detection Range:

  0.16-10 μg/mL

• Sensitivity:

  41 ng/mL